Capable of handling large sample volumes
The CytoCatch™ Isolation Platform is capable of processing large sample volumes, up to 15 mL, which is optimal due to the extremely low concentration of CTCs in blood.
Cytocatch Isolation Platform
Backed by
Enabling high performance isolation of CTCs
The Cytocatch™ technology has outstanding recovery rates, with yields above 97% when processing blood samples spiked with tumor cells from prostate, breast, lung, and colorectal cancer cell lines.
Label-free isolation of CTCs
Stop losing valuable information and forget about the limitations of capturing CTC subpopulations with diminished expression of epithelial markers. The capture approach employed by the CytoCatch™ Isolation Platform is based on the differences in size and deformability between blood cells and CTCs. This allows the capture of CTCs whose epithelial phenotype is downregulated, in comparison to technologies that rely on specific antigen-antibody interactions, which typically use EpCAM antibodies to selectively isolate CTCs.
Fully automated
The CytoCatch™ Isolation Platform automatically performs the necessary steps to process the sample and subsequently stain the collected cells with fluorescently labeled antibodies, increasing the reliability and reproducibility of the assay by preventing human errors and cell loss due to manual steps.
Customize your experiments
Its powerful software enables the construction of custom processing protocols in a quick and easy manner without previous programming knowledge. Customize all the steps executed by the CytoCatch™ Isolation Platform during and after the processing of the sample.
Always reproducible
The CytoCatch™ Isolation Platform integrates multiple sensors and control algorithms to guarantee the correct processing of the sample, and hence ensure that the results are always reproducible.
Features
No sample pre-enrichment required
Unlike other technologies, there is no need to perform any tedious pre-enrichment steps before the processing of the sample, avoiding the inherent cell loss related to these procedures.
Exceptionally fast
The time required to process a 15 mL blood sample is approximately 20 minutes.
Downstream applications
Compatible with a broad range of analysis techniques
The captured CTCs are compatible with a broad range of analysis techniques enabling DNA, RNA, and protein-based molecular profiling.
Traditional & next generation sequencing
Immunocytochemistry
PCR assays and its variants
Microarrays
Ex vivo CTC culture
Functional analyses
Specifications
Technical overview
A complete technical snapshot of the CytoCatch™ Isolation Platform — sample handling, capture, automation and downstream compatibility.
Sample handling
- Sample type
- Whole blood (EDTA or CellSave preserved)
- Sample volume
- Up to 15 mL per run
- Pre-enrichment
- Not required
- Throughput
- ≈ 20 minutes per sample
Capture & detection
- Capture mode
- Label-free, size- and deformability-based
- Recovery rate
- > 97% across prostate, breast, lung & colorectal cell lines
- EpCAM-independent
- Yes — captures CTCs with downregulated epithelial phenotype
- On-chip staining
- Automated immunofluorescent labeling
Automation & reliability
- Operation
- Fully automated end-to-end workflow
- Process control
- Multi-sensor monitoring with feedback algorithms
- Custom protocols
- Drag-and-drop protocol builder, no programming required
- Reproducibility
- Sensor-validated each run
Downstream compatibility
- Output
- Captured CTCs ready for direct downstream analysis
- Compatible assays
- NGS, ICC, PCR, microarrays, ex vivo culture, functional analyses
- Connectivity
- Pairs with the CytoCatch™ Imaging System for full digital workflow